期刊
ANNALS OF THE RHEUMATIC DISEASES
卷 73, 期 1, 页码 284-289出版社
BMJ PUBLISHING GROUP
DOI: 10.1136/annrheumdis-2012-202878
关键词
Arthritis; Chondrocytes; Knee Osteoarthritis; Osteoarthritis; Treatment
类别
资金
- Big Lottery Fund through the AKU Society
- MRC
- Rosetrees Trust
Background Alkaptonuria (AKU) is a rare metabolic disease caused by deficiency of homogentisate 1,2 dioxygenase, an enzyme involved in tyrosine catabolism, resulting in increased circulating homogentisic acid (HGA). Over time HGA is progressively deposited as a polymer (termed ochronotic pigment) in collagenous tissues, especially the cartilages of weight bearing joints, leading to severe joint disease. Objectives To characterise blood biochemistry and arthropathy in the AKU mouse model (Hgd-/-). To examine the therapeutic effect of long-term treatment with nitisinone, a potent inhibitor of the enzyme that produces HGA. Methods Lifetime levels of plasma HGA from AKU mice were measured by high-performance liquid chromatography (HPLC). Histological sections of the knee joint were examined for pigmentation. The effect of nitisinone treatment in both tissues was examined. Results Mean (SE) plasma HGA levels were 3- to 4-fold higher (0.148 +/- 0.019mM) than those recorded in human AKU. Chondrocyte pigmentation within the articular cartilage was first observed at 15weeks, and found to increase steadily with mouse age. Nitisinone treatment reduced plasma HGA in AKU mice throughout their lifetime, and completely prevented pigment deposition. Conclusions The AKU mouse was established as a model of both the plasma biochemistry of AKU and its associated arthropathy. Early-stage treatment of AKU patients with nitisinone could prevent the development of associated joint arthropathies. The cellular pathology of ochronosis in AKU mice is identical to that observed in early human ochronosis and thus is a model in which the early stages of joint pathology can be studied and novel interventions evaluated.
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