期刊
PHYTOTHERAPY RESEARCH
卷 20, 期 2, 页码 115-119出版社
WILEY
DOI: 10.1002/ptr.1812
关键词
flavonoid; silydianin; oxidative stress; apoptosis; polymorphonuclear neutrophils
The inhibitory effect of silydianin, an active constituent of Silybium marianum, on the in vitro production and release of oxidative products has been examined. Polymorphonuclear neutrophils (PMNs) play a primary role in the initiation and propagation of inflammatory responses. Their apoptosis is a major mechanism associated with the resolution of inflammatory reactions. Neutrophils were assessed for caspase-3 activity, the first step in the execution phase of apoptosis. When cells were cultured with 100 gm silydianin for 24 h, caspase-3 was activated. Induction of apoptosis by silydianin was accompanied by a decrease in luminol-enhanced chemiluminescence as well as superoxide radical (O-2(center dot)-) release in freshly isolated cells and lipid peroxidation in mouse spleen microsomes. No significant effect of silydianin on PMN hydrogen peroxide production evaluated by a flow cytometric dichlorofluorescin oxidation assay was found. Such results indicate a possible antiinflammatory activity for silydianin, which regulates caspase-3 activation, affects cell membranes and acts as a free radical scavenger. Copyright (c) 2006 John Wiley & Sons, Ltd.
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