期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 23, 期 2, 页码 411-420出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msj046
关键词
apurinic/apyrimidinic endonuclease; L1Tc; NARTc; non-LTR retrotransposon; retroposon; site specificity; Trypanosoma cruzi
资金
- NIAID NIH HHS [U01 AI045038, AI45038, AI43062] Funding Source: Medline
The trypanosomatid protozoan Trypanosoma cruzi Contains long autonomous (L1Tc)and short nonautonomous (NARTc) non-long terminal repeat retrotransposons. NARTc (0.25 kb) probably derived from LITc (4.9 kb) by 3'-deletion. It has been proposed that their apparent random distribution in the genome is related to the L1Tc-encoded apurinic/apyrimidinic endonuclease (APE) activity, which repairs modified residues. To address this question we used the T. cruzi (CL-Brener strain) genome data to analyze the distribution of all the L1Tc/NARTc elements present in contigs larger than 10 kb. This data set, which represents 0.91 X sequence coverage of the haploid nuclear genome (similar to 55 Mb), contains 419 elements, including 112 full-length L1Tc elements (14 of which are potentially functional) and 84 full-length NARTc. Approximately half of the full-length elements are flanked by a target site duplication, most of them (87%) are 12 bp long. Statistical analyses of sequences flanking the full-length elements show the same highly conserved pattern upstream of both the L1Tc and NARTc retrotransposons. The two most conserved residues are a guanine and an adenine, which flank the site where first-strand cleavage is performed by the element-encoded endonuclease activity. This analysis clearly indicates that the L1Tc and NARTc elements display relative site specificity for insertion, Which suggests that the APE activity is not responsible for first-strand cleavage of the target site.
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