Lysinibacillus cresolivorans sp nov., an m-cresol-degrading bacterium isolated from coking wastewater treatment aerobic sludge

A Gram-stain-positive, rod-shaped, facultatively anaerobic, endospore-forming bacterium (designated strain SC03(T)) was isolated from the aerobic treatment sludge of a coking plant (Shaoguan City, China). The optimal pH and temperature for growth were pH 7.0 and 35 degrees C. On the basis of 16S rRNA gene sequence analysis, strain SC03(T) was related to the genus Lysinibacillus and the similarity between strain SC03(T) and the most closely related type strain, Lysinibacillus macroides LMG 18474(T), was 94.4 %. The genomic G+C content of the DNA of strain SC03(T) was 41.2 mol%. Chemotaxonomic data supported the affiliation of strain SC03(T) to the genus Lysinibacillus. These properties include MK-7 as the predominant menaquinone; iso-C-15 : (0) and iso-C-16 : (0) as major fatty acids; A4 alpha(L-Lys-D-Asp) as the cell-wall peptidoglycan type; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine plus three unknown phospholipids as polar lipids. The phenotypic, phylogenetic and chemotaxonomic characters enable the differentiation of strain SC03(T) from recognized Lysinibacillus species. Thus, strain SC03(T) represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus cresolivorans sp. nov. is proposed. The type strain is SC03(T) (=NRRL B-59352(T)=CCTCC M 208210(T)).