期刊
FEMS MICROBIOLOGY LETTERS
卷 255, 期 2, 页码 308-315出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2005.00089.x
关键词
AFM; atomic force microscopy; cell morphology; cell physiology; ethanol stress; yeasts
类别
The detrimental effects of ethanol toxicity on the cell surface morphology of Saccharomyces cerevisiae (strain NCYC 1681) and Schizosaccharomyces pombe (strain DVPB 1354) were investigated using an atomic force microscope (AFM). In combination with culture viability and mean cell volume measurements AFM studies allowed us to relate the cell surface morphological changes, observed on nanometer lateral resolution, with the cellular stress physiology. Exposing yeasts to increasing stressful concentrations of ethanol led to decreased cell viabilities and mean cell volumes. Together with the roughness and bearing volume analyses of the AFM images, the results provided novel insight into the relative ethanol tolerance of S. cerevisiae and Sc. pombe.
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