4.4 Article

Analysis of the lateral flagellar gene system of Aeromonas hydrophila AH-3

期刊

JOURNAL OF BACTERIOLOGY
卷 188, 期 3, 页码 852-862

出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.188.3.852-862.2006

关键词

-

向作者/读者索取更多资源

Mesophilic Aeromonas strains express a polar flagellum in all culture conditions, and certain strains produce lateral flagella on semisolid media or on surfaces. Although Aeromonas lateral flagella have been described as a colonization factor, little is known about their organization and expression. Here we characterized the complete lateral flagellar gene cluster of Aeromonas hydrophila AH-3 containing 38 genes, 9 of which (lafA-U) have been reported previously. Among the flg(L), and lafA structural genes we found a modification accessory factor gene (maf-5) that is involved in formation of lateral flagella; this is the first time that such a gene has been described for lateral flagellar gene systems. All Aeromonas lateral flagellar genes were located in a unique chromosomal region, in contrast to Vibrio parahaemolyticus, in which the analogous genes are distributed in two different chromosomal regions. In A. hydrophila mutations in flhA(L), lajK, fliJ(L), flgN(L), flgE(L), and maf-5 resulted in a loss of lateral flagella and reductions in adherence and biofilm formation, but they did not affect polar flagellum synthesis. Furthermore, we also cloned and sequenced the A. hydrophild AH-3 alternative sigma factor sigma(54) (rpoN); mutation of this factor suggested that it is involved in expression of both types of flagella.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据