期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 831, 期 1-2, 页码 303-306出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2005.12.018
关键词
E-6-O-p-coumaroyl scandoside methyl ester; p-coumaric acid; pharmacokinetics; metabolite
A rapid and simple high-performance. liquid chromatographic (HPLC) method has been developed for the determination of p-coumaric acid in rat plasma and applied to a pharmacokinetic study in rats after administration of a prodrug, E-6-O-p-coumaroyl scandoside methyl ester, isolated from Hedyotis diffusa (Willd.). Sample preparation involved protein precipitation with acetonitrile. The supernatant was then injected onto a DiamonsilTM C-18 column (250 mm x 4.6 min i.d., 5 mu m). The mobile phase consisted of acetonitrile-water (21:79, v/v) with 1% glacial acetic acid. The UV detector was set at 310 run. The lower limit of quantification of p-coumaric acid in rat plasma was 0.02 mu g/mL. The calibration curves were linear over the concentration range 0.02-5 mu g/mL with correlations greater than 0.999. The assay procedure was applied to the study of the metabolite phari-nacokinetics of E-6-O-p-coumaroyl scandoside methyl ester in rat. (c) 2005 Elsevier B.V. All rights reserved.
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