期刊
CARBOHYDRATE POLYMERS
卷 63, 期 2, 页码 188-197出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2005.08.002
关键词
polysaccharides; cytokine; perforin; granzyme B; antitumour activity; immunomodulatory
Ganoderma lucidum polysaccharides (Gl-PS) have shown a variety of immune modulating effects. Culture of immunologic effector cells termed cytokine induced killer (CIK) cells is dependent on exogenous cytokines. It is not known about effects of Gl-PS on cytokine production, perforin and granzyme B expression in CIK cells. We made use of CIK cells as a means to investigate interaction between Gl-PS and cytokines, and explore mechanism of Gl-PS acting on proliferation and anti-tumor activity of CIK cells. The results suggested that Gl-PS (400 or 100 mu g/ml) promoting CIK cells proliferation and cytotoxicity were relevant to enhancing IL-2, TNF production, protein and mRNA expression of granzyme B and perforin in CIK cells through synergizing cytokines in decreasing doses of IL-2 and anti-CD3 by 75 and 50%, and maybe were irrelevant to nitric oxide (NO). These results confirmed that Gl-PS was a promising biological response modifier (BRM) and immune potentiator. (c) 2005 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据