期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 355, 期 5, 页码 957-967出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2005.11.029
关键词
protein transport; Tat pathway; twin-arginine; thylakoid membrane; translocation intermediate
In the thylakoid membrane of chloroplasts as well as in the cytoplasmic membrane of bacteria, the Delta pH/Tat-dependent protein transport pathway is responsible for the translocation of folded proteins. Using the chimeric 16/23 protein as model substrate in thylakoid transport experiments, we dissected the transport process into several distinct steps that are characterized by specific integral translocation intermediates. Formation of the early translocation intermediate Ti-1, which still exposes the N and the C terminus to the stroma, is observed with thylakoids pretreated with (i) solutions of chaotropic salts or alkaline pH, (ii) protease, or (iii) antibodies raised against TatA, TatB, or TatC. Membrane insertion takes place even into liposomes, demonstrating that proteinaceous components are not required. This suggests that Tat-dependent transport may be initiated by the unassisted insertion of the substrate into the lipid bilayer, and that interaction with the Tat translocase takes place only in later stages of the process. (c) 2005 Elsevier Ltd. All rights reserved.
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