期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 54, 期 3, 页码 823-828出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf0522715
关键词
blotransformation; flavonolds; O-methyltransfe rase; Streptomyces avermitilis
O-Methylation, commonly found in synthesis of secondary metabolites of plants and micro-organisms, appears to transfer a methyl group to the hydroxyl group of the recipient which increases the hydrophobicity of the recipient. O-Methyltransf erase (OMT), SaOMT-2, was isolated and characterized from Streptomyces avermitilis MA-4680. Its amino acid sequence showed 68% similarity with antibiotic C-1027 OMT and 53% similarity with the carminomycin 4-OMT. SaOMT-2 was expressed in E coli as a His-tag fusion protein and showed that the methyl was transferred onto the 7-hydroxyl group of the isoflavones, daidzein and genistein, and the flavones, kaempferol and quercetin, as well as the flavanone naringenin. NMR and liquid chromatography-mass spectrometry were used to confirm the location of the methyl group on the recipient compound of naringenin, which was biotransformed into sakuranetin by E coli transformant expressing SaOMT-2 (E. coli Sa-2). Therefore, E coli Sa-2 would be used for the synthesis of the antifungal flavonoid, sakuranetin, through biotransformation.
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