期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 110, 期 5, 页码 2387-2392出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp055370u
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资金
- NCRR NIH HHS [P41 RR008119, RR-08119, P41 RR008119-13, P41 RR008119-14] Funding Source: Medline
- NHGRI NIH HHS [R01 HG002655-06, R01 HG002655-04A1, R01 HG002655-05, HG-02655, R01 HG002655] Funding Source: Medline
- NIBIB NIH HHS [R01 EB000682, R01 EB000682-04, R01 EB000682-05, EB-00682] Funding Source: Medline
N-(2-Mercaptopropionyl)glycine (tiopronin)-coated silver nanoparticles (average diameter of metal core = 5 nm) were prepared by a modified Brust method. Tiopronin ligands were partially displaced by thiolate single-stranded oligonucleotides via ligand exchange. These particles were immobilized onto a solid substrate through hybridization with target oligonucleotides in a layer-by-layer approach. The dye-labeled complementary oligonucleotides were bound to the particle layers through hybridization. Fluorescence intensity was enhanced with a simultaneous increase of plasmon absorbance from accumulated particles. A steady state was shown at the 10th particle layer and then the fluorescence enhancement showed a plateau. This result reveals that increasing the particle layer contributes to fluorescence enhancement. This novel method was used to detect DNA hybridization through both absorbance and emission spectral changes.
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