期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1105, 期 1-2, 页码 71-76出版社
ELSEVIER
DOI: 10.1016/j.chroma.2005.12.001
关键词
column-switching; capillary liquid chromatography; restricted access media (RAM); drug analysis; sample preparation automation
This study presents the application of a system that joins the known advantages of capillary liquid chromatography (e.g., higher concentration of the analytes and lower consumption of mobile phase) with those of column-switching using restricted access material (RAM) (sample clean up and extraction) to the analysis of fluoxetine in plasma samples. Automatically, the system loads the biological sample, while a RAM-BSA-C18 column (50 mm x 520 mu m) excludes the macromolecules and focuses the analytes; afterwards, a second mobile phase elutes the analytes, in backflush mode, and provides the separation in a C18 analytical column (100 mm x 520 mu m). We optimized the procedure for a total analysis time of 25 min. Using this approach the calibration curve shows r = 0.998 with a linearity range from 20 to 500 ng ml(-1). Precision, calculated as relative standard deviation (RSD), was < 20%. The developed miniaturized system showed to be adequate and attractive, demonstrating a large potential for sample preparation. (c) 2005 Elsevier B.V. All rights reserved.
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