Nocardioides antarcticus sp nova, isolated from marine sediment

Strain M-SA3-94(T), an aerobic, Gram-stain-positive, ovoid- to rod-shaped, non-motile bacterium, was isolated from the marine sediment of Ardley cove, King George Island, Antarctica. Strain M-SA3-94(T) grew optimally at pH 5.0-6.0, 20 degrees C and in the presence of 1.0% (w/v) NaCl. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain M-SA3-94(T) belonged to the genus Nocardioides in the family Nocardioidaceae, clustering with Nocardioides plantarum NCIMB 12834(T), Nocardioides ginsengagri BX5-10(T), Nocardioides marinquilinus CL-GY44(T) and Nocardioides lianchengensis D94-1(T) (with 96.1 %, 95.9%, 94.5% and 94.7% 16S rRNA gene sequence similarity, respectively). The chemotaxonomic properties of strain M-SA3-94(T) were similar to those of members of the genus Nocardioides with validly published names. The major fatty acid was iso-C-16:0. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol and three unknown phospholipids. The diagnostic diamino acid in the cell-wall peptidoglycan was LL-2, 6-diaminopimelic acid. MK-8(H4) was the predominant menaquinone and the DNA G+C content of this strain was 66.7 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic data, strain M-SA3-94(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides antarcticus sp. nov. is proposed. The type strain is M-SA3-94(T) (=CCTCC AB2014053(T)=LMG 28254(T)).