Mameliella phaeodactyli sp.. nov., a member of the family Rhodobacteraceae isolated from the marine algae Phaeodactylum tricomutum

A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, yellow bacterium designated strain KD53(T), was isolated from a culture of the alga Phaeodactylum tricomutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD53(T) was a member of the Roseobacter clade within the family Rhodobacteraceae, forming a distinct lineage with species of the genus Mameliella. The 16S rRNA gene sequence similarities between strain KD53(T) and other strains examined were all less than 97.0 %. Strain KD53(T) was found to grow optimally at 28 degrees C, at pH 7.5-8.0 and in the presence of 3% (w/v) NaCl. The dominant fatty acids of strain KD53(T) were C-(18:1)omega 6c and/or C-18:1 omega 7c, C-18:0 and C-16:0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 65 mol% and the major respiratory quinone was ubiquinone 10 (Q-10). On the basis of phenotypic data and phylogenetic inference, strain KD53(T) represents a novel member of the genus Mameliella, then the name Mameliella phaeodactyli sp. nov. is proposed. The type strain is KD53(T) (=MCCC 1K00273(T)=KCTC 42178(T)).