Brain dysmyelination and recovery assessment by noninvasive in vivo diffusion tensor magnetic resonance imaging

Diffusion tensor magnetic resonance imaging (DT-MRI) was applied for in vivo quantification of myelin loss and regeneration. A transgenic mouse line (Oligo-TTK) expressing a truncated form of the herpes simplex virus 1 thymidine kinase gene (hsv1-tk) in oligodendrocytes was studied along with two induced phenotypes of myelin pathology. Myelin loss and axonal abnormalities differentially affect values of DT-MRI parameters in the brain of transgenic mice. Changes in the anisotropy of the white matter were assessed by calculating and mapping the radial (D-perpendicular to) and axial (D-parallel to) water diffusion to axonal tracts and fractional anisotropy (FA). A significant increase in D-perpendicular to attributed to the lack of myelin was observed in all selected brain white matter tracts in dysmyelinated mice. Lower D-parallel to values were consistent with the histological observation of axonal modifications, including reduced axonal caliber and overexpression of neurofilaments and III p-tubulin. We show clearly that myelination and axonal changes play a role in the degree of diffusion anisotropy, because FA was significantly decreased in dysmyelinated brain. Importantly, myelin reparation during brain postnatal development induced a decrease in the magnitude of D-perpendicular to and an increase in FA compared with the same brain before recovery. The progressive increase in D-parallel to values was attributed to the gain in normal axonal morphology. This regeneration was confirmed by the detection of enlarged oligodendrocyte population, newly formed myelin sheaths around additional axons, and a gradual increase in axonal caliber. (c) 2006 Wiley-Liss, Inc.