期刊
BIOCHEMICAL JOURNAL
卷 394, 期 -, 页码 325-334出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20050834
关键词
cardiomyocytes; CTP : phosphocholine cytidylyltransferase (CTP); ischaemia; necrosis; phosphatidylcholine (PtdCho); phospholipid bilayer
A decrease in [H-3]Cho(choline) incorporat ion in to PtdCho (phospliatidylcholine) preceded the onset of LDH (lactate dehydrogenase) release in HL-1 cardiomyocytes Submitted to simulated ischaemia. This observation led LIS to examine the role of PtdCho synthesis in sarcolemmal disruption in HL-1 cardiomyocytes. To address this objective we analysed the individual effects of hypoxia, glucose deprivation and acidosis, three prominent components of ischamia, on the different steps of the Kennedy pathway for the synthesis of PtdCho. Pulse and pulse-chase experiments with [H-3]Cho, performed in whole HL-1 cells submitted to hypoxia or normoxia, in the presence or absence of glucose at different pHs indicated first, that CK (choline kinase) was inhibited by hypoxia and acidosis, whereas glucose deprivation exacerbated the inhibition Caused by hypoxia. Second, the rate-limiting reaction in PtdCho synthesis, catalysed by CCT (CTP:phosphocholine cytidylyltransferase), was inhibited by hypoxia and glucose deprivation, but unexpectedly activated by acidosis. In cell-free system assays, acidosis inhibited both CK and CCT. In experiments performed in whole cells, the effect of acidosis was likely to be direct oil CK, but indirect or intact-cell-dependent on CCT. Since hypoxia and glucose deprivation favoured membrane disruption, but acidosis prevented it, we hypothesized that the modulation of CCT could be all important determinant of cell Survival. Supporting this hypothesis, we show that CCT activity in whole-cell experiments clearly correlated with LDH release, but riot with ATP concentration. Altogether Our results Suggest a significant role for CCT activity in sarcolemmal disruption during ischaemia.
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