期刊
BIOSENSORS & BIOELECTRONICS
卷 21, 期 8, 页码 1434-1442出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2005.06.004
关键词
biosensor; filamentous bacteriophage; phage display; piezoelectric; quartz crystal microbalance; Salmonella typhimurium
类别
资金
- NIAID NIH HHS [1 R21 AI055645] Funding Source: Medline
Proof-in-concept biosensors were prepared for the rapid detection of Salmonella typhimurium in solution, based on affinity-selected filamentous phage prepared as probes physically adsorbed to piezoelectric transducers. Quantitative deposition studies indicated that approximate to 3 x 10(10) phage particles/cm(2) could be irreversibly adsorbed for I h at room temperature to prepare working biosensors. The quality of phage deposition was monitored by fluorescent microscopy. Specific-bacterial binding resulted in resonance frequency changes of prepared sensors, which were evaluated using linear regression analysis. Sensors possessed a rapid response time of < 180 s, had a low-detection limit of 10(2) cells/ml and were linear over a range of 10(1)-10(7) cells/ml with a sensitivity of 10.9 Hz per order of magnitude of S. typhimurium concentration. Viscosity effects due to increasing bacterial concentration and non-specific binding were not significant to the piezoelectric platform as confirmed by dose-response analysis. Phage-bacterial binding was confirmed by fluorescence and scanning electron microscopy. Overall, phage may constitute effective bioreceptors for use with analytical platforms for detecting and monitoring bacterial agents, including use in food products and possibly biological warfare applications. (c) 2005 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据