期刊
BIOPOLYMERS
卷 81, 期 3, 页码 194-201出版社
WILEY
DOI: 10.1002/bip.20399
关键词
DNA; T-tetrad; oligonucteotide; quadruplex; bunch-ODN
The solid phase syntheses of the bunch oligonucleotides 1 and 2 based on the sequences of the natural oligodeoxynucleotides (ODNs) d(TG(2)TG(2)C) (3) and d(CG(2)TG(2)T) (4), respectively, attached to a non-nucleotidic tetrabranched linker, are reported. Bunch-ODNs 1 and 2 were shown to form more stable monomolecular parallel G-quadruplexes I and H when compared with their tetramolecular counterparts [d(TG(2)TG(2)C)](4) (III) and [d(CG(2)TG(2)T)](4) (IV), respectively. The structure and stability of all the synthesized complexes have been investigated by circular dichroism (CD), CD thermal denaturation experiments, and H-1-NMR (nuclear magnetic resonance) experiments at variable temperatures. Particularly, the spectroscopic data confirmed that 1 adopts a T-tetrad containing parallel-stranded quadruplex structure I as in the tetramolecular complex III. (c) 2005 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据