期刊
VIROLOGY
卷 345, 期 2, 页码 317-327出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2005.09.066
关键词
M-PMV; retrovirus; assembly; gag; immature capsid; internal scaffold domain; plasma membrane
类别
资金
- NIAID NIH HHS [R01 AI043230-05, AI-43230] Funding Source: Medline
The Mason-Pfizer monkey virus (M-PMV) Gag protein follows a morphogenesis pathway in which immature capsids are preassembled within the cytoplasm before interaction with and budding through the plasma membrane. Intracytoplasmic assembly is facilitated by sequences within the p12 domain of Gag that we have termed the Internal Scaffold Domain (ISD). If M-PMV utilizes all ISD then what provides the equivalent function for most other retroviruses that assemble at the plasma membrane? To investigate the possibility that the membrane itself fulfills this role, we have combined functional deletion of the ISD with a Mutation that disrupts intracellular targeting or with a plasma membrane targeting signal. By either modification, targeting of ISD-deleted Gag to the plasma membrane restores particle production. These results provide Support for a model in which the plasma membrane and the D-type ISD provide all interchangeable scaffold-like function in retrovirus assembly. (c) 2005 Elsevier Inc. All rights reserved.
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