期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 113, 期 2, 页码 730-748出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2005.07.025
关键词
explosives; competitive immunoassay; self-assembled monolayers; quartz crystal microbalance; surface plasmon resonance
This contribution describes the synthesis, characterisation and evaluation of a novel biochip technology for the detection of the explosive substance 2,4,6-trinitrotoluene (TNT). Two types of thiols are self-assembled to produce the biochip on gold, namely oligo(ethylene glycol) (OEG)-alkyl thiols terminated with a hydroxyl group and a TNT-analogue (2,4-dinitrobenzene), respectively. Three different TNT-analogues are mixed in various proportions with hydroxyl-terminated OEG-thiols to obtain highly selective and sensitive biochips with a low non-specific binding. The produced self-assembled monolayers (SAMs) are thoroughly characterised with null ellipsometry, contact angle goniometry, infrared reflection absorption spectroscopy (IRAs) and X-ray photoelectron spectroscopy (XPS) and they all meet high standards in terms of molecular conformation, packing and orientation. The biochip, is designed to function as a platform for a competitive label-free immunoassay and two real-time transducers-surface plasmon resonance (SPR) and quartz crystal microbalance (QCM)-are used to monitor the dissociation of on-line immobilised monoclonal antibodies produced against TNT. The three TNT-analogues are all potential candidates for the development of a functional biochip, though one of them displayed superior properties in terms of shorter recovery/stabilisation time after antibody immobilisation and a better response/loading capacity ratio. This is particularly evident when using low antigen (TNT-analogue) content in the mixed SAM. (c) 2005 Elsevier B.V. All rights reserved.
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