期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 101, 期 2, 页码 149-155出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762006000200006
关键词
oral fluid; viral hepatitis; diagnostic and epidemiology
In this report, we examine the adaptability of commercially available serological kits to detect antibodies markers for viral hepatitis in oral fluid samples. We also assessed the prevalence of hepatitis A, B, and C virus-specific antibodies, and related risk factors for these infectious diseases through sensitivity of the tests in saliva samples to evaluate if oral fluid can be an alternative tool to substitute serum in diagnosis of acute viral hepatitis and in epidemiological studies. One hundred and ten paired serum and saliva specimens from suspect patients Of having acute hepatitis were collected to detect antibodies to hepatitis A (total and IgM) hepatitis B (anti-HBs, total anti-HBc and IgM anti-HBc), and hepatitis C (anti-HCV) using commercially available enzyme-linked immunossorbent assay (EIA). In relation to serum samples, oral fluid assay sensitivity and specificity were as follows: 87 and 100% for total anti-HAV 79 and 100% for anti-HAV IgM, 6 and 95% foranti-HBs, 13 and,100% for total anti-HBc, 100 and 100% for anti-HBc IgM, and 75 and 100% for anti-HCV The consistency observed between antibodies tests in saliva and expected risk factors for hepatitis A and C suggests that the saliva method could replace serum in epidemiological studies for hepatitis A and C.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据