期刊
JOURNAL OF MASS SPECTROMETRY
卷 41, 期 3, 页码 372-389出版社
WILEY-BLACKWELL
DOI: 10.1002/jms.997
关键词
quadrupole time-of-flight mass spectrometry; multiple precursor ion scanning; linear ion trap-orbitrap mass spectrometry; MS fragmentation; inositol-containing sphingolipids; Saccharonlyces cerevisiae; Schizosaccharomyces pombe; Pichia pastoris
The yeast Saccharomyces cerevisiae synthesizes three classes of sphingolipids: inositolphosphoceramides (IPCs), mannosyl-inositolphosphoceramides (MIPCs), and mannosyl-diinositolphosphoceramides (M(IP)(2)C). Tandem mass spectrometry of their molecular anions on a hybrid quadrupole time-of-flight (QqTOF) instrument produced fragments of inositol-containing head groups, which were specific for each lipid class. MSn analysis performed on a hybrid linear ion trap-orbitrap (LTQ Orbitrap) mass spectrometer with better than 3 ppm mass accuracy identified fragment ions specific for the amide-linked fatty acid and the long chain base moieties in individual molecular species. By selecting m/z of class-specific fragment ions for multiple precursor ion scanning, we profiled yeast sphingolipids in total lipid extracts on a QqTOF mass spectrometer. Thus, a combination of QqTOF and LTQ Orbitrap mass spectrometry tends itself to rapid, comprehensive and structure-specific profiling of the molecular composition of sphingolipids and glycerophospholipids in important model organisms, such as fungi and plants. Copyright (c) 2006 John Wiley & Sons, Ltd.
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