期刊
GENETICS
卷 172, 期 3, 页码 1427-1439出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.105.051698
关键词
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资金
- NIGMS NIH HHS [R01 GM028216, GM28216] Funding Source: Medline
The Saccharomyces casein kinase 1 isoforms encoded by the essential gene pair YCK1 and YCK2 control cell growth and morphogenesis and are linked to the endocytosis of several membrane proteins. Here we define roles for the Yck1,2 kinases in Mal61p maltose permease activation and trafficking. using I yck1 Delta, yck2-2(ts) (yck(ts)) strain with conditional Yck activity Moreover, we provide evidence that Glc7-Reg1 phosphatase acts as an upstream activator of Yck1,2 kinases in a novel signaling pathway that Modulates kinase activity in response to carbon source availability The yck(ts) strain exhibits significantly reduced maltose transport activity despite apparently normal levels and Cell Surface localization of maltose permease protein. Glucose-induced internalization and rapid loss of maltose transport. activity of Mal61/HAp-GFP are not observed in the yck(ts) strain and maltose permease proteolysis is blocked. We show that a reg1 Delta, mutant exhibits a phenotype remarkably similar to that conferred by yck(ts). The reg1 Delta, phenotype is not enhanced in the yck(ts) reg1 Delta double mutant. and is suppressed by increased Yck1,2p dosage. Further, although Yck2p localization and abundance do not change in the reg1 Delta mutant, Yckl,2 kinase activity as assayed by glucose-induced HXT1 expression and Mth1 repressor stability, is substantially reduced in the reg1 Delta, strain.
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