Preconceptional fasting of fathers alters serum glucose in offspring of mice

Objective: Maternal nutrition has long-term effects on offspring characteristics. Similar effects mediated through fathers have not been tested. Methods: Outbred Swiss male mice were fasted one or six times 1 to 4 wk before mating. Offspring were killed at age intervals of 4 to 10 wk and their sera were analyzed for glucose, corticosterone, and insulin-like growth factor-1. Statistical linear mixed effects models were used to determine treatment (paternal diet restriction versus control) differences and possible effects of covariates, including sex, litter membership, and litter size. Results: Paternal food deprivation resulted in a consistent decrease in average serum glucose in male and female offspring. Significant changes in corticosterone and insulin-like growth factor-1 were found for some groups. The results indicated a male-mediated transgenerational effect on metabolism- and growth-related parameters, in particular glucose. Conclusions: Effects of paternal nutritional experiences on offspring metabolism, if confirmed, would be novel and could have far-reaching implications in the context of transgenerational effects on chronic diseases. (c) 2006 Elsevier Inc. All rights reserved.