期刊
EUROPEAN JOURNAL OF NUTRITION
卷 45, 期 2, 页码 97-104出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00394-005-0569-4
关键词
vitamin C; antioxidant; double-blind placebo-controlled trial; DNA damage
Background Antioxidants are believed to prevent many types of disease. Some previous studies suggest that dietary supplementation with vitamin C results in a decrease in the level of one of the markers of oxidative damage-8-oxoguanine in the DNA of peripheral blood mononuclear cells (PBMC). Aim of the trial To investigate the effect of different dose levels of dietary supplementation with vitamin C on oxidative DNA damage. Methods A randomised double-blind placebo-controlled trial was carried out using three different levels (80, 200 and 400 mg) of dietary vitamin C supplementation in a healthy population of 160 volunteers; supplementation was for a period of 15 weeks followed by a 10 week washout period. Peripheral blood samples were obtained every 5 weeks from baseline to 25 weeks. Results An increase in PBMC vitamin C levels was not observed following supplementation in healthy volunteers. There was no effect found on 8-oxoguanine measured using HPLC with electrochemical detection for any of the three supplemented groups compared to placebo. 8-oxoadenine levels were below the limit of detection of the HPLC system used here. Conclusions Supplementation with vitamin C had little effect on cellular levels in this group of healthy individuals, suggesting their diets were replete in vitamin C. The dose range of vitamin C used did not affect oxidative damage in PBMC DNA.
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