期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 72, 期 3, 页码 2221-2225出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.72.3.2221-2225.2006
关键词
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The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.
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