期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 70, 期 3, 页码 740-742出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70.740
关键词
Bacillus subtilis; conjugational transfer; pLS20
pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.
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