期刊
FEMS MICROBIOLOGY ECOLOGY
卷 55, 期 3, 页码 339-350出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1574-6941.2005.00040.x
关键词
bacterial community; 16S rRNA; DGGE; global change; carbon dioxide; FACE
类别
The response of total (DNA-based analysis) and active (RNA-based analysis) bacterial communities to a pCO(2) increase under field conditions was assessed using two perennial grasses: the nitrophilic Lolium perenne and the oligonitrophilic Molinia coerulea. PCR- and reverse transcriptase-PCR denaturing gradient gel electrophoresis analysis of 16S rRNA genes generated contrasting profiles. The pCO(2) increase influenced mainly the active and root-associated component of the bacterial community. Bacterial groups responsive to the pCO(2) increase were identified by sequencing of corresponding denaturing gradient gel electrophoresis bands. About 50% of retrieved sequences were affiliated to Proteobacteria. Our data suggest that Actinobacteria in soil and Myxococcales (Deltaproteobacteria) in root are stimulated under elevated pCO(2).
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