期刊
PROTEIN ENGINEERING DESIGN & SELECTION
卷 19, 期 3, 页码 135-140出版社
OXFORD UNIV PRESS
DOI: 10.1093/protein/gzj008
关键词
artificial protein; frame shift mutation; in vitro evolution; microgene; Y-family DNA polymerase
We describe 'frame shuffling', a novel method for preparing artificial protein libraries. With this method, a Y-family DNA polymerase known to introduce frame shift mutations at high rates is utilized to scramble the reading frames of a parental gene. The resultant progeny produce mutant proteins having segmental sequence changes. Such frame-shuffled mutant proteins exhibit physicochemical properties that differ from those of proteins obtained using conventional mutagenesis.
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