期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 46, 期 1, 页码 16-22出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2005.07.026
关键词
edible vaccine; Escherichia coli heat-labile toxin; fusion protein; mucosal carrier; Nicotiana tabacum; porcine epidemic diarrhea virus
The pentameric B subunit of Escherichia coli heat-labile enterotoxin (LTB) can be used as an efficient mucosal carrier of either immunogenic or tolerogenic T-cell epitopes. Co-delivery of therapeutic proteins with carrier proteins could increase the effectiveness of the antigen. This paper reports the ability of transgenic tobacco plants to express a fusion protein consisting of the synthetic LTB and a synthetic neutralizing epitope of porcine epidemic diarrhea virus (PEDV), causing an enteric disease that is especially severe in piglets. Both components of the fusion proteins were detected in Western blot analysis, and binding assay confirmed that plant-synthesized pentameric LTB-PEDV fusion bound to the intestinal membrane GMI-ganglioside receptor. This suggested that the fusion protein retained both its native antigenicity and the ability to form pentamers. (c) 2005 Elsevier Inc. All rights reserved.
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