期刊
ANALYTICAL BIOCHEMISTRY
卷 350, 期 1, 页码 52-60出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2005.11.036
关键词
resonance energy transfer; redox state; midpoint potential; azurin; cytochrome; flavodoxin
A method for fluorescence detection of a protein's redox state based on resonance energy transfer from an attached fluorescence label to the prosthetic group of the redox protein is described and tested for proteins containing three types of prosthetic groups: a type-l copper site (azurin, amicyanin, plastocyanin, and pseudoazurin), a heme group (cytochrome c550), and a flavin mononucleotide (flavodoxin). This method permits one to reliably distinguish between reduced and oxidized proteins and to perform potentiometric titrations at submicromolar concentrations. (c) 2005 Elsevier Inc. All rights reserved.
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