期刊
PROTEIN ENGINEERING DESIGN & SELECTION
卷 19, 期 3, 页码 107-111出版社
OXFORD UNIV PRESS
DOI: 10.1093/protein/gzj009
关键词
mosquito; protein engineering
资金
- NIAID NIH HHS [R01 AI 29092] Funding Source: Medline
The Bacillus thuringiensis crystal protein Cry1Aa is normally selectively active to caterpillar larvae. Through rational design, toxicity (mu g/ml) to the mosquito Culex pipiens was introduced by selected deletions and substitutions of the loop residues of domain II. Toxicity to its natural target Manduca sexta was concomitantly abolished. The successful grafting of the alternate mosquito toxicity onto the original lepidopteran Cry1Aa toxin demonstrates the possibility of designing and engineering a desired toxicity into any toxin of a common scaffold by reshaping the receptor binding region with desired specificities.
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