期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 384, 期 6, 页码 1308-1313出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-005-0275-3
关键词
multi-wall carbon nanotubes; chemically modified electrode; high-performance liquid chromatography; dopamine; microdialysis
High performance liquid chromatography coupled with microdialysis sampling and electrochemical detection (HPLC-ECD) has been used to determine dopamine (DA). In the HPLC-ECD a multi-wall carbon nanotube electrode chemically modified with carboxyl groups (MWNT-COOH CME) was used as the working electrode for determination of DA. The results indicated that the MWNT-COOH CME enabled efficient electrocatalytic oxidation of DA with relatively high sensitivity and stability and long life. Peak currents for dopamine were linearly dependent on concentration in the range 5.0x10(-9) to 5.0x10(-5) mol L-1 and the calculated detection limit (S/N=3) was 2.5x10(-9) mol L-1. The method had been successfully used to measure dopamine in rat striatal microdialysate. To study the physiological effect of nitric oxide (NO) on striatal release of DA, 0.5 mmol L-1 sodium nitroprusside (SNP) was a continuously perfused into rat striatum. This resulted in a 46% increase in DA basal level.
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