期刊
NATURE NEUROSCIENCE
卷 9, 期 3, 页码 443-452出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nn1654
关键词
-
资金
- NICHD NIH HHS [5T32HD007032] Funding Source: Medline
- NIDA NIH HHS [P50 DA005010, P50DA05010] Funding Source: Medline
- NINDS NIH HHS [U24 NS43562, 5T32NS048004, NS047391, NS049501] Funding Source: Medline
A major challenge in systems neuroscience is to perform precise molecular genetic analyses of a single neuronal population in the context of the complex mammalian brain. Existing technologies for profiling cell type-specific gene expression are largely limited to immature or morphologically identifiable neurons. In this study, we developed a simple method using fluorescent activated cell sorting (FACS) to purify genetically labeled neurons from juvenile and adult mouse brains for gene expression profiling. We identify and verify a new set of differentially expressed genes in the striatonigral and striatopallidal neurons, two functionally and clinically important projection neuron subtypes in the basal ganglia. We further demonstrate that Ebf1 is a lineage-specific transcription factor essential to the differentiation of striatonigral neurons. Our study provides a general approach for profiling cell type-specific gene expression in the mature mammalian brain and identifies a set of genes critical to the function and dysfunction of the striatal projection neuron circuit.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据