期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 63, 期 3, 页码 424-429出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2005.02.006
关键词
depuration; microcystins; tilapia; fish; bile; excretion
Previous studies demonstrated that microcystins (MCYSTs) can be accumulated in different organs, particularly the liver, of tilapia fish (Oreochromis niloticus) in an Egyptian fish farm containing toxic blooms of Microcystis aeruginosa. In the present study, a microcosm experiment was conducted to examine the deputation of MCYSTs in tilapia fish from this fish farm. Fish were grown in a 100-L aerated recirculation tank containing dechlorinated water at room temperature (25 +/- 2 degrees C) for 96h. MCYST concentrations in livers, intestines, and gallbladders of each daily sacrificed fish were determined by both enzyme-linked immunosorbent assay (ELISA) and protein phosphatase inhibition assay (PPIA). MCYST concentrations in the surrounding water were also determined by the same methods. The results showed that MCYST concentrations in the liver and intestine decreased gradually throughout the experimental period. This decrease was accompanied by an increase in MCYST concentrations in the gallbladder and surrounding water. The maximum value of MCYST in the surrounding water was obtained after 96 h at a level of 1.2 mu g/L by ELISA, while it was obtained after 24h at a level of 0.5 mu g/L by PPIA and remained stable until the end of the experiment. The present study revealed that tilapia fish can depurate and excrete MCYSTs into the bile and surrounding water as a way to avoid toxicity from such a potent hepatotoxin. (c) 2005 Elsevier Inc. All rights reserved.
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