Isolation and characterization of 15 microsatellite loci in the poplar rust fungus, Melampsora larici-populina, and cross-amplification in related species

We developed 15 microsatellite loci in the poplar rust fungus, Melampsora larici-populina, using two enrichment protocols. Polymorphism of each locus was assessed on a panel of 30 isolates, comprising three subpanels (world, regional and local scales). Thirteen loci were polymorphic with three to eight alleles detected. The 15 loci were also tested on five related Melampsora species, M. allii-populina, M. medusae f. sp. deltoidae, M. larici-tremulae, M. rostrupii and M. pinitorqua, and partial or global cross-amplification events were detected.