期刊
JOURNAL OF VIROLOGY
卷 80, 期 5, 页码 2515-2528出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.80.5.2515-2528.2006
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资金
- FIC NIH HHS [D43 TW000231, D43 TW00231] Funding Source: Medline
- NIAID NIH HHS [AI058763, R01 AI100790, R01 AI055461, R37 AI033292, AI49566, R56 AI058763, R01 AI058763, AI33292, AI055461, R01 AI033292] Funding Source: Medline
Human immunodeficiency virus type I (HIV-1) neutralizing antibodies are thought be distinguished from nonneutralizing antibodies by their ability to recognize functional gp120/gp41 envelope glycoprotein (Env) trimers. The antibody responses induced by natural HIV-1 infection or by vaccine candidates tested to date consist largely of nonneutralizing antibodies. One might have expected a more vigorous neutralizing response, particularly against virus particles that bear functional trimers. The recent surprising observation that nonneutralizing antibodies can specifically capture HIV-1 may provide a clue relating to this paradox. Specifically, it was suggested that forms of Env, to which nonneutralizing antibodies can bind, exist on virus surfaces. Here, we present evidence that HIV-1 particles bear nonfunctional gp120/gp41 monomers and gp120-depleted gp41 stumps. Using a native electrophoresis band shift assay, we show that antibody-trimer binding predicts neutralization and that the nonfunctional forms of Env may account for virus capture by nonneutralizing antibodies. We hypothesize that these nonfunctional forms of Env on particle surfaces serve to divert the antibody response, helping the virus to evade neutralization.
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