Blood plasma antioxidant defense in patients with pancreatitis

Objectives: The aim of this study was to establish the main determinants of plasma antioxidant potential in different types of human pancreatitis: chronic pancreatitis (CP, n=19), chronic pancreatitis exacerbated (CPE, n=16), and acute pancreatitis (AP, n=8). Methods: We measured the plasma thiobarbituric acid reactive substances (TBARS), total peroxyl radical trapping potential (TRAP), and the levels of antioxidants such as uric acid (UA), SH groups (SH), ascorbic acid (AA), and bilirubin (BIL). Results: In comparison to healthy volunteers (HV, n=22) the average levels of TBARS in all types of pancreatitis were significantly higher (by 50% 90%), and plasma SH groups were lower (similar to 30%). In the multivariate regression models, the variability of plasma TRAP was explained in the HV group through UA only (36%). in the CP group through UA, AA, and BIL together (75%), and in CPE group through UA and AA together (81%). Plasma SH concentration did not enter the regression models in all groups. The AP group revealed paradoxically high concentrations of AA and TRAP and a negative, linear correlation between the level of TRAP versus UA concentration (R=-0.87). Conclusions: The multivariate regression analysis points to different structures of antioxidant defense in healthy people and those with pancreatitis: they were much the same in the CP and CPE groups but fundamentally different in the AP group. AP may activate unidentified antioxidant defenses as an adaptive response against oxidative stress.