期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 447, 期 1, 页码 46-52出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2006.01.005
关键词
acyl-CoA synthetase; vectorial acylation; fatty acid transport; rat; yeast; triglyceride synthesis
资金
- NIDDK NIH HHS [DK59935] Funding Source: Medline
- NIGMS NIH HHS [GM56850] Funding Source: Medline
Mammals express multiple isoforms of acyl-CoA synthetase (ACSL1 and ACSL3-6) in various tissues. These enzymes are essential for fatty acid metabolism providing activated intermediates for complex lipid synthesis, protein modification, and P-oxidation. Yeast in contrast express four major ACSLs, which have well-defined functions. Two, Faa1p and Faa4p, are specifically required for fatty acid transport by vectorial acylation. Four ACSLs from the rat were expressed in a yeast faa1 Delta faa4 Delta strain and their roles in fatty acid transport and trafficking characterized. All four restored ACS activity yet varied in substrate preference. ACSL1, 4, and 6 were able to rescue fatty acid transport activity and triglyceride synthesis. ACSL5, however, was unable to facilitate fatty acid transport despite conferring robust oleoyl-CoA synthetase activity. This is the first study evaluating the role of the mammalian ACSLs in fatty acid transport and supports a role for ACSL1, 4, and 6 in transport by vectorial acylation. (c) 2006 Elsevier Inc. All rights reserved.
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