期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 70, 期 3, 页码 654-659出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70.654
关键词
glucose sensor; glucose dehydrogenase; FAD; amperometric sensor
A novel FAD-dependent glucose, dehydrogenase (FAD-GDH) was found and its enzymatic property for glucose sensing was characterized. FAD-GDH oxidized glucose in the presence of some artificial electron acceptors, except for O-2, and exhibited thermostability, high substrate specificity and a large Michaelis constant for glucose. FAD-GDH was applied to an amperometric glucose sensor with Fe(CN)(6)(3-) as a soluble mediator. The use of a relatively high concentration of Fe(CN)(6)(3-) resulted in a good linearity between the current response and the glucose concentration, taking into account a large Michaelis constant for Fe(CN)(6)(3-). The glucose sensor was completely insensitive to 02 and responded linearly to glucose up to 30 mm. Compared to glucose, the response to other saccharides was negligible. The sensor can be stored at room temperature in a desiccator for at least one month without any change in the response or activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据