期刊
GLYCOBIOLOGY
卷 16, 期 3, 页码 237-243出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwj058
关键词
carbohydrate antigen; galectin; hepatic granulomas; schistosomiasis
Schistosomes, major parasitic helminths, express numerous glycoconjugates that provoke humoral and cellular immune responses in the infected human host. The main pathology in schistosomiasis is due to the formation of granulomas around tissue-trapped eggs and the resulting organ damage. By using a mouse model of induction of granulomas by hepatic implantation of antigen-coated beads, it has been determined that the glycan part of schistosomal soluble egg antigens (SEA) initiates granulomogenesis. To identify which individual glycan elements in this complex SEA mixture are granulomogenic, we have tested in the same mouse model conjugates of various synthetic oligosaccharides characteristic for schistosome eggs, including GalNAc beta 1-4GlcNAc (LacdiNAc, LDN), Gal beta 1-4(Fuc alpha 1-3)GlcNAc (Lewis(x)), Fuc alpha 1-2Fuc alpha 1-3GlcNAc (DF-Gn), and Fuc alpha 1-3GalNAc beta 1-4(Fuc alpha 1-3)GlcNAc (F-LDN-F). Ribonuclease (RNase) A and B, and different fetuin glycoforms were included as controls. Only beads that carry glycoconjugates with terminal LacdiNAc or Gal beta 1-4GlcNAc (LacNAc, LN) elements gave rise to granulomas, with macrophage, lymphocyte, and eosinophil levels similar to the granulomatous lesions caused by schistosome eggs in a natural infection. Uncoated beads, and beads coated with fucosylated glycoconjugates or glycoconjugates lacking terminally exposed Gal or GalNAc, only attracted a monolayer of macrophages. These results indicate that the formation of hepatic granulomas is triggered specifically by glycoconjugates which carry terminal LacNAc or LacdiNAc, both constituents of the schistosome egg.
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