期刊
ACTA VETERINARIA BRNO
卷 75, 期 1, 页码 99-106出版社
VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO
DOI: 10.2754/avb200675010099
关键词
acute toxicity; haematological profile; biochemical profile of blood; histological examination of tissues
The aim of the study was to investigate acute toxicity of clove oil for European catfish and, using values of haematological and biochemical profiles of blood and histological tissue examinations, to assess the effects of the fish exposure to that anaesthetic. Acute toxicity values of clove oil for European catfish found were 10minLC50 76.70 mg(.)l(-1); 10minLC0.1 49.60 mg(.)l(-1); 10minLC99.9 118.50 mg(.)l(-1); 96hLC50 18.40 mg(.)l(-1); 96hLC0.1 10.70 mg(.)l(-1); and 96hLC99.9 31.90 mg(.)l(-1). Individual phases of anaesthesia and recovery were determined. The 10-min exposure to clove oil at a concentration of 30 mg(.)l(-1) caused a significant ( p < 0.05) increase in the concentration of triacylglycerols ( TRIG), alanin aminotransferase ( ALT) and decreased ( p < 0.05) in mean corpuscular haemoglobin concentration ( MCHC) immediately after anaesthesia. The leukocyte counts were significantly ( p < 0.05) decreased 24 hours after anaesthesia. A significant ( p < 0.05) decrease of percentage distribution lymphocytes was found immediately after anaesthesia. On the other hand, percentage and absolute count of myeloid cells were increased. Increased percentage count of eosinophils outlasted 24 hours after anaesthesia, absolute counts of these cells were consistents with control. Histological examination showed capillary ectasia of gill filaments immediately after clove oil anaesthesia. Twenty-four hours after anaesthesia, no ectasia was observed. No histopathological changes were demonstrated in other tissues following anaesthesia. Results of the examinations suggest that the use of clove oil at a concentration of 30 mg(.)l(-1) does not cause irreversible damage in European catfish.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据