期刊
JOURNAL OF NEURO-ONCOLOGY
卷 77, 期 1, 页码 25-32出版社
SPRINGER
DOI: 10.1007/s11060-005-9010-3
关键词
malignant glioma; nuclear factor-kappaB; syndecan; syndecan-1
Invasion of tumor cells into the surrounding normal brain tissues is a prominent feature of malignant gliomas. Malignant glioma cells secrete thrombospondin-1 which participates in the motility of glioma cells and binds cell surface heparan sulfate proteoglycan. To clarify the invasion mechanism of tumor cells, expression of the syndecans (syndecan-1, -2, -3, and -4), a major cell surface heparan sulfate proteoglycan family, was analyzed in malignant gliomas. Involvement of nuclear factor-kappaB (NF-kappa B) on syndecan-1 expression was also investigated. Using reverse transcription-PCR, the authors analyzed the expression of syndecan-1 -2. -3. and -4 in 10 malignant glioma cell lines, 2 glioblastoma specimens, and 2 normal brain specimens. All malignant glioma cell lines and glioblastoma specimens expressed all types of syndecan mRNA, except in one glioma cell line that lacked syndecan-3 expression. On the other hand, normal brain specimens expressed syndecan-2, -3, and -4 mRNA, but did not syndecan-1 mRNA. Syndecan-1 protein was localized in the cell surface of all malignant glioma cell lines by flow cytometry. Various levels of active nuclear factor-kappa B (NF-kappa B) was detected in all malignant glioma cell lines using immunoblotting. The expression of active NF-kappa B and syndecan-1 increased in U251 glioma cells after tumor necrosis factor-alpha or interleukin-1 beta treatment, which can activate NF-kappa B. The amplification of active NF-kappa B and syndecan-1 by tumor necrosis factor-alpha or interleukin-1 beta was suppressed by an inhibitor of NF-kappa B activation (emodin). Emodin also downregulated the expression of syndecan-1 mRNA in U251 cells. These results indicate that malignant glioma cells express all types of syndecans and suggest that NF-kappa B participates in the upregulation of the syndecan-1 expression at the transcriptional level, and increased expression of syndecan-1 could associate with extracellular matrices including thrombospondin-1.
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