期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 95, 期 3, 页码 1491-1498出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00697.2005
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资金
- NIDDK NIH HHS [DK-55619, DK-64566] Funding Source: Medline
Pharmacological manipulation of fatty acid metabolism in the hypothalamic arcuate nucleus (ARC) alters energy balance and glucose homeostasis. Thus, we tested the hypotheses that distinctive populations of ARC neurons are oleic acid (OA) sensors that exhibit a glucose dependency, independent of whether some of these OA sensors are also glucose-sensing neurons. We used patch-clamp recordings to investigate the effects of OA on ARC neurons in brain slices from 14- to 21-day-old Sprague - Dawley (SD) rats. Additionally, we recorded spontaneous discharge rate in ARC neurons in 8-wk-old fed and fasted SD rats in vivo. Patch-clamp studies showed that in 2.5 mM glucose 12 of 94 (13%) ARC neurons were excited by 2 mu M OA (OA-excited or OAE neurons), whereas six of 94 (6%) were inhibited (OA-inhibited 2.5 or OAI(2.5) neurons). In contrast, in 0.1 mM glucose, OA inhibited six of 20 (30%) ARC neurons ( OAI(0.1) neurons); none was excited. None of the OAI(0.1) neurons responded to OA in 2.5 mM glucose. Thus OAI(2.5) and OAI(0.1) neurons are distinct. Similarly, in seven of 20 fed rats (35%) the overall response was OAE-like, whereas in three of 20 (15%) it was OAI-like. In contrast, in fasted rats only OAI-like response were observed ( three of 15; 20%). There was minimal overlap between OA-sensing neurons and glucose-sensing neurons. In conclusion, OA regulated three distinct subpopulations of ARC neurons in a glucose-dependent fashion. These data suggest that an interaction between glucose and fatty acids regulates OA sensing in ARC neurons.
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