期刊
ULTRAMICROSCOPY
卷 106, 期 4-5, 页码 376-382出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultramic.2005.11.001
关键词
three-dimensional reconstruction; resolution; depth of field; FREALIGN
类别
资金
- NIGMS NIH HHS [1 P01 GM-62580] Funding Source: Medline
Most algorithms for three-dimensional (3D) reconstruction from electron micrographs assume that images correspond to projections of the 3D structure. This approximation limits the attainable resolution of the reconstruction when the dimensions of the structure exceed the depth of field of the microscope. We have developed two methods to calculate a reconstruction that corrects for the depth of field. Either method applied to synthetic data representing a large virus yields a higher resolution reconstruction than a method lacking this correction. (c) 2005 Elsevier B.V. All rights reserved.
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