期刊
ANALYTICA CHIMICA ACTA
卷 561, 期 1-2, 页码 178-182出版社
ELSEVIER
DOI: 10.1016/j.aca.2006.01.011
关键词
molecularly imprinted polymer; sinomenine; in situ technique; application
A sinomenine (SIN) molecularly imprinted polymer (MIP) with specific recognition ability for SIN was prepared by in situ molecularly imprinted technique. The polymer was then packed into a polypropylene column and used as a solid-phase extraction (SPE) sorbent. Utilizing this MIP-SPE as the separation medium, a crude extract of SIN from Sinoinenium acutum Rcht. et Wils was purified, and relatively pure SIN was obtained. At the same time, based on the specific molecular recognition ability of MIP, a determination method of SIN in blood was established with MIP as the separation medium. The linear range was 5-60 mu g ml(-1), and the linear correlation coefficient of the standard curve was 0.9975, The average recovery was 98%, with the relative standard deviation (R.S.D.) 7% (within-day) and 8% (between-day). These results reveal that the established method can be used for analysis of SIN in biological samples. (c) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据