期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 10, 页码 3890-3895出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0508214103
关键词
gene profiling; gene regulation; neuronal differentiation; retina; transcription factor
资金
- NEI NIH HHS [EY11115, EY14259, T32 EY013934-01A1, EY13934, T32 EY013934, R01 EY014259-01, F31 EY007003, R01 EY011115, R01 EY011115-04, EY07003, R01 EY014259, P30 EY007003] Funding Source: Medline
- NIGMS NIH HHS [R01 GM067840] Funding Source: Medline
The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation of the Nrl gene in mice leads to functional cones at the expense of rods. We show that a 2.5-kb Nrl promoter segment directs the expression of enhanced GFP specifically to rod photoreceptors and the pineal gland of transgenic mice. GFP is detected shortly after terminal cell division, corresponding to the timing of rod genesis revealed by birthdating studies. In Nrl(-/-) retinas, the GFP+ photoreceptors express S-opsin, consistent with the transformation of rod precursors into cones. We report the gene profiles of freshly isolated flow-sorted GFP+ photoreceptors from wild-type and Nrl(-/-) retinas at five distinct developmental stages. Our results provide a framework for establishing gene regulatory networks that lead to mature functional photoreceptors from postmitotic precursors. Differentially expressed rod and cone genes are excellent candidates for retinopathies.
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