期刊
VETERINARY MICROBIOLOGY
卷 113, 期 1-2, 页码 83-87出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2005.10.014
关键词
morbillivirus; peste-des-petits ruminants virus; vaccine virus; M gene; nucleotide sequence; amino acid sequence
The M gene nucleotide sequence of an Indian peste-des-petits ruminants (PPRV) vaccine virus (PPRV Sungri/96) belonging to Asian lineage was determined. The gene is 1476 nucleotides long with a single open reading frame (ORF). The nucleotide and predicted amino acid sequence was compared with the homologous region of the African Lineage Vaccine virus PPRV/Nigeria/75/1. The nucleotide sequence of the PPRV Sungri/96 was 86% identical to that of PPRV/Nigeria/75/1, while a homology of 93% and 95% could be observed in the ORF and amino acids level, respectively. The M gene encodes a protein of 335 amino acids, with a predicted molecular weight (MW) of 37.8 kDa. The ORF is flanked by a 3' untranslated region of 436 nucleotides and a high level of sequence divergence (similar to 30%) could be observed in this region between the vaccine viruses of Asian and African lineages. A high degree of conservation of several amino acids of this protein observed previously was also confirmed in this study. (C) 2005 Elsevier B.V.. All rights reserved.
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