期刊
AQUATIC TOXICOLOGY
卷 76, 期 3-4, 页码 321-328出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquatox.2005.10.009
关键词
olfactory receptor neurons; degeneration; regeneration; copper; fish; Tilapia
Low levels of Cu2+ are known to specifically cause olfactory neuron death in fish olfactory epithelium. This study investigated the morphological changes in the olfactory mucosa of the cichlid Tilapia mariae, after a 4-day exposure to different concentrations of Cu2+ (20, 40 and 100 mu g/l), and the regeneration time-frame, when fish exposed to 20 mu g/l were returned to dechlorinated tap water. Light microscopy, combined with Fluoro Jade-B staining, permitted the observation of a dose-dependent damage which became less severe and more circumscribed to receptor cells when Cu2+ concentration decreased. The regeneration process in the olfactory tissue was examined in fish after 0, 3, and 10 days of recovery in well water. Immunostaining with PCNA showed a massive mitotic activity in the basal region of the mucosa immediately after exposure was terminated. The mitotically produced elements were immature neurons since they expressed the neural growth-associated phosphoprotein GAP-43. After 3 days of recovery the nuclei had already completed their migration to the upper portion of the epithelium and mitotic activity was much less intensive. After 10 days the olfactory tissue did not present differences when compared to the control tissue. These results suggest that after 10 days the regeneration is completed and the integrity of the tissue restored. (C) 2005 Elsevier B.V. All rights reserved.
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