期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 447, 期 2, 页码 97-106出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2006.02.002
关键词
vitamin E; nuclear receptors; PPAR gamma; transglutaminase-1; keratinocytes; differentiation
In skin, vitamin E acts as the predominant lipophilic antioxidant with a protective function against irradiation and oxidative stress. In addition to that, vitamin E can also modulate signal transduction and gene expression. To study whether the four natural tocopherol analogues (alpha, beta-, gamma-, delta-tocopherol) can influence transcriptional activity by modulating the activity of nuclear receptors, a human keratinocytes cell line (NCTC 2544) was transfected with plasmids containing the luciferase reporter gene under control by direct repeat elements (DR1-DR4), representing binding sites for four different classes of nuclear receptors. In this model, the tocopherols positively modulated only the reporter construct containing a consensus element for peroxisome proliferator-activated receptors (PPARs). The induction was strongest with gamma-tocopherol and was most likely the direct consequence of stimulation of PPAR gamma protein expression in keratinocytes. Vitamin E treatment also led to increased expression of a known PPAR gamma target gene involved in terminal keratinocytes differentiation, the transglutaminase-1. (c) 2006 Elsevier Inc. All rights reserved.
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